Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography–mass spectrometry analysis
Identifieur interne : 000121 ( France/Analysis ); précédent : 000120; suivant : 000122Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography–mass spectrometry analysis
Auteurs : Gabrielle Beaumier-Gallon [France] ; Christophe Dubois [France] ; Henri Portugal [France] ; Denis Lairon [France]Source :
- Atherosclerosis [ 0021-9150 ] ; 1998.
English descriptors
- KwdEn :
Abstract
We hypothesized that intestinal absorption and postprandial re-secretion of dietary cholesterol may be a particularly complex process in humans. To test this hypothesis, we used deuterium-enriched cholesterol to specifically label meal cholesterol and developed an improved method for quantitative measurement of traces of deuterated cholesterol as well as cholesterol with reference to two different internal standards by gas chromatography–mass spectrometry (GC–MS) measurement. In the first study, a group of healthy subjects ingested a single test meal containing deuterated cholesterol with a 7 h postprandial follow-up. In the second one, a group of healthy subjects ingested a first test meal containing deuterated cholesterol and a follow-up was performed during three consecutive test meals and later until 72 h. The most striking observations were that the occurrence of dietary cholesterol in chylomicrons is not concomitant to triglycerides and is very low after a single meal while most dietary cholesterol is re-secreted in chylomicrons after a second, and even a third, fat test meal. The data obtained show that the re-secretion of dietary cholesterol from the small intestine is a slow and complex process in humans.
Url:
DOI: 10.1016/S0021-9150(98)00223-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000677
- to stream Istex, to step Curation: 000538
- to stream Istex, to step Checkpoint: 002767
- to stream Main, to step Merge: 003060
- to stream Main, to step Curation: 002F77
- to stream Main, to step Exploration: 002F77
- to stream France, to step Extraction: 000121
Links to Exploration step
ISTEX:21A3E9C0E25DEA94E695536E1BB58E865D5BEBB0Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography–mass spectrometry analysis</title><author><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name></author><author><name sortKey="Dubois, Christophe" sort="Dubois, Christophe" uniqKey="Dubois C" first="Christophe" last="Dubois">Christophe Dubois</name></author><author><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name></author><author><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:21A3E9C0E25DEA94E695536E1BB58E865D5BEBB0</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0021-9150(98)00223-8</idno><idno type="url">https://api.istex.fr/document/21A3E9C0E25DEA94E695536E1BB58E865D5BEBB0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000677</idno><idno type="wicri:Area/Istex/Curation">000538</idno><idno type="wicri:Area/Istex/Checkpoint">002767</idno><idno type="wicri:doubleKey">0021-9150:1998:Beaumier Gallon G:postprandial:studies:on</idno><idno type="wicri:Area/Main/Merge">003060</idno><idno type="wicri:Area/Main/Curation">002F77</idno><idno type="wicri:Area/Main/Exploration">002F77</idno><idno type="wicri:Area/France/Extraction">000121</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography–mass spectrometry analysis</title><author><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unit 476, Human Nutrition and Lipids at INSERM (National Institute of Health and Medical Research), 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Dubois, Christophe" sort="Dubois, Christophe" uniqKey="Dubois C" first="Christophe" last="Dubois">Christophe Dubois</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unit 476, Human Nutrition and Lipids at INSERM (National Institute of Health and Medical Research), 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Department of Analytical Chemistry, Faculty of Pharmacy, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unit 476, Human Nutrition and Lipids at INSERM (National Institute of Health and Medical Research), 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Atherosclerosis</title><title level="j" type="abbrev">ATH</title><idno type="ISSN">0021-9150</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">141</biblScope><biblScope unit="supplement">S1</biblScope><biblScope unit="page" from="S81">S81</biblScope><biblScope unit="page" to="S85">S85</biblScope></imprint><idno type="ISSN">0021-9150</idno></series><idno type="istex">21A3E9C0E25DEA94E695536E1BB58E865D5BEBB0</idno><idno type="DOI">10.1016/S0021-9150(98)00223-8</idno><idno type="PII">S0021-9150(98)00223-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9150</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cholesterol</term><term>Diet</term><term>Gas chromatography–mass spectrometry</term><term>Human</term><term>Lipoproteins</term><term>Postprandial lipemia</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We hypothesized that intestinal absorption and postprandial re-secretion of dietary cholesterol may be a particularly complex process in humans. To test this hypothesis, we used deuterium-enriched cholesterol to specifically label meal cholesterol and developed an improved method for quantitative measurement of traces of deuterated cholesterol as well as cholesterol with reference to two different internal standards by gas chromatography–mass spectrometry (GC–MS) measurement. In the first study, a group of healthy subjects ingested a single test meal containing deuterated cholesterol with a 7 h postprandial follow-up. In the second one, a group of healthy subjects ingested a first test meal containing deuterated cholesterol and a follow-up was performed during three consecutive test meals and later until 72 h. The most striking observations were that the occurrence of dietary cholesterol in chylomicrons is not concomitant to triglycerides and is very low after a single meal while most dietary cholesterol is re-secreted in chylomicrons after a second, and even a third, fat test meal. The data obtained show that the re-secretion of dietary cholesterol from the small intestine is a slow and complex process in humans.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name></region><name sortKey="Dubois, Christophe" sort="Dubois, Christophe" uniqKey="Dubois C" first="Christophe" last="Dubois">Christophe Dubois</name><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MozartV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000121 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000121 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MozartV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:21A3E9C0E25DEA94E695536E1BB58E865D5BEBB0
|texte= Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography–mass spectrometry analysis
}}
| This area was generated with Dilib version V0.6.20. |  |